Inhibition activity of spirulina platensis proteins photo-immobilization biomaterial on proliferation of cancer cells
Guan Y, Guo B.
Biotechnology Research Institute, South China Normal University, Guangzhou, China.
The bioactive protein-phycocyanin and all the proteins of Spirulina Platensis were isolated and purified. Photo-reactive proteins were synthesized by coupling the proteins with (N-(4-azidobenzoyloxy)succinimide) and were spread onto the 24-well cell culture polystyrene plate. Then the coated surface was exposed to ultraviolet irradiation for chemical fixation of proteins via the conversion of the phenylazido group to the highly reactive phenyl-nitrene which spontaneously formed covalent bonds with neighboring hydrocarbons. On these proteins-immobilized polystyrene plates, the liver cancer cells 7402 were cultured under the serum-free conditions, and the inhibition activity on proliferation of liver cancer cells was investigated and analyzed.
PUB: Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2002 Jan;19(1):1-3.